Journal article
Novel whole cell adhesion assays of three isolates of the fouling diatom Amphora coffeaeformis reveal diverse responses to surfaces of different wettability
OM Hodson, JP Monty, PJ Molino, R Wetherbee
Biofouling | TAYLOR & FRANCIS LTD | Published : 2012
Abstract
Whole cell, strength of adhesion assays of three different isolates of the fouling diatom Amphora coffeaeformis were compared using a hydrophilic surface viz. acid washed glass (AWG), and a hydrophobic surface viz. a self assembled monolayer (SAM) of undecanethiol (UDT). Assays were performed using a newly designed turbulent flow channel that permits direct observation and recording of cell populations on a test surface. Exposure to continuous shear stress over 3 h revealed that the more motile isolate, WIL2, adhered much more strongly to both test surfaces compared to the other two strains. When the response of the isolates to shear stress after 3 h was compared, there was no significant di..
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Awarded by Australian Research Council
Funding Acknowledgements
The authors thank Roland Szymanski and Wayne Powrie of the Physics Workshop, The University of Melbourne, for helping to design, and for the construction of, the flow channel. Wayne Powrie provided assistance with maintenance of the flow channel. The Australian Research Council (ARC Linkage Grant LP0454982), and International Paint (Akzo Nobel), Gateshead, UK, provided funding for this project. The authors also thank Mr John Lewis, Ms Lyn Fletcher, Dr Andrew Scardino and colleagues from the Australian Defence Science and Technology Organisation (DSTO) for their financial and technical assistance in facilitating this study.